Maintaining the Stability of Placental RNA in Normal Conditions

Submit Manuscript | http://medcraveonline.com extraction of RNA in particular mRNA which is protein coding [1]. The RNA in total need to be protein free, intact, free of inhibitor enzymes which could interfere with the downstream segments of transcripts [2,3]. Hence, the quality, purity and integrity of extracted RNAs beside the quantity are the values which are under improvement of developing kits for this purpose. Application of RNA latter (Qiagen, www.qiagen.com)for tissue immersion and consequence storage at 4°C,-20°C and -80°C while in 1 mL of TRIzol Reagent (Invitrogen, www.invitrogen. com); also, flash-frozen in liquid nitrogen and storage at -80 °C until tissue processing have been numerous methods to achieve an optimum way for preservation to keep the entire tissue nature and entity with no degradation in RNA property [4]. We investigated the importance of storage procedure for solid tissue immediately after excision from the body.